Skip to main content
Addgene

pCB116
(Plasmid #53365)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS416
  • Backbone size w/o insert (bp) 5580
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cup9
  • Alt name
    YPL177C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    940
  • GenBank ID
    855926
  • Entrez Gene
    CUP9 (a.k.a. YPL177C)
  • Promoter GAL1
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13rev
  • 3′ sequencing primer M13pUC-Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB116 was a gift from Alexander Varshavsky (Addgene plasmid # 53365 ; http://n2t.net/addgene:53365 ; RRID:Addgene_53365)

  • For your References section:

    The N-end rule pathway controls the import of peptides through degradation of a transcriptional repressor. Byrd C, Turner GC, Varshavsky A. EMBO J. 1998 Jan 2. 17(1):269-77. 10.1093/emboj/17.1.269 PubMed 9427760
Related items: