pExo1
(Plasmid #53357)

• Purpose: Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.
• Depositing Lab: Lingchong You
• Publication: Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19810-5. doi: 10.1073/pnas.1211072109. Epub 2012 Nov 9.

Backbone

• Vector backbone: pPROLar.A122
• Backbone manufacturer: Clonetech

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JM109
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: BlaMs
• Insert Size (bp): 792
• Promoter: p(lac/ara-1)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unkown
• 3′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: hlyAs
• Insert Size (bp): 657
• Promoter: p(lac/ara-1)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry