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Addgene

GST-PRDM16 1-223
(Plasmid #53346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-2
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDM16
  • Alt name
    PR domain containing 16
  • Species
    M. musculus (mouse)
  • Mutation
    aa 1-223
  • Entrez Gene
    Prdm16 (a.k.a. 5730557K01Rik, csp1, mel1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRDM16 1-223 was a gift from Shingo Kajimura & Bruce Spiegelman (Addgene plasmid # 53346 ; http://n2t.net/addgene:53346 ; RRID:Addgene_53346)

  • For your References section:

    Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex. Kajimura S, Seale P, Tomaru T, Erdjument-Bromage H, Cooper MP, Ruas JL, Chin S, Tempst P, Lazar MA, Spiegelman BM. Genes Dev. 2008 May 15;22(10):1397-409. doi: 10.1101/gad.1666108. 10.1101/gad.1666108 PubMed 18483224
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