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Addgene

pET16b-alpha3D
(Plasmid #53310)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5711
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha3D
  • Alt name
    a synthetic antiparallel coiled coil
  • Species
    Synthetic
  • Insert Size (bp)
    219
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-alpha3D was a gift from William DeGrado (Addgene plasmid # 53310 ; http://n2t.net/addgene:53310 ; RRID:Addgene_53310)

  • For your References section:

    Solution structure and dynamics of a de novo designed three-helix bundle protein. Walsh ST, Cheng H, Bryson JW, Roder H, DeGrado WF. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5486-91. PubMed 10318910
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