pCGN-HCF-1 fl
(Plasmid #53309)

• Purpose: plasmid contains human HCFC1 full length gene with HA and c-myc tags
• Depositing Lab: Winship Herr
• Publication: Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 5063
• Total vector size (bp): 11252
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HCFC1
• Alt name: HCF-1
• Alt name: HCF-1 fl
• Species: H. sapiens (human)
• Insert Size (bp): 6105
• GenBank ID: L20010.1
• Entrez Gene: HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • c-myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for
• 3′ sequencing primer: -

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry