pCGN-Oct-1
(Plasmid #53308)

• Purpose: plasmid contains POU2F1 (Oct-1) gene with HA-tag
• Depositing Lab: Winship Herr
• Publication: Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 5063
• Total vector size (bp): 7277
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct-1
• Alt name: POU2F1
• Alt name: OTF-1
• Alt name: OBP100, NFIII
• Species: H. sapiens (human)
• Insert Size (bp): 2229
• GenBank ID: X13403
• Entrez Gene: POU2F1 (a.k.a. OCT1, OTF1, Oct1Z, oct-1B)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for
• 3′ sequencing primer: -

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry