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NELFE-mOrange pHis-//
(Plasmid #53303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis-parallel
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NELF-E protein
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Nelf-E (a.k.a. Dmel_CG5994, CG5994, Dmel\CG5994, NELF, NELF-E, Nelf-e, anon-66Da, cg5994, dNelf-E)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • mOrange (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NELFE-mOrange pHis-// was a gift from John Lis (Addgene plasmid # 53303 ; http://n2t.net/addgene:53303 ; RRID:Addgene_53303)

  • For your References section:

    Comprehensive analysis of RNA-protein interactions by high-throughput sequencing-RNA affinity profiling. Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT. Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 2014 May 8. 10.1038/nmeth.2970 PubMed 24809628
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