mCit-Snap25b D179K A199R
(Plasmid
#53259)
-
PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepECFP-C1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5333
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSNAP-25B
-
Alt namesynaptosomal-associated protein, 25kDa
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)621
-
MutationD179K and A199R
-
Entrez GeneSnap25 (a.k.a. SNAP-25B, SNAP-25a)
- Promoter CMV
-
Tags
/ Fusion Proteins
- mCitrine (N terminal on backbone)
- 4aa-linker (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This SNAP25B double mutant is resistant to both botulinum toxin C1 (A199R) and botulinum toxin E (D179K)
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mCit-Snap25b D179K A199R was a gift from Robert Zucker (Addgene plasmid # 53259 ; http://n2t.net/addgene:53259 ; RRID:Addgene_53259) -
For your References section:
Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses. Degtyar V, Hafez IM, Bray C, Zucker RS. J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013. 10.1523/JNEUROSCI.2337-12.2013 PubMed 23536066
Map uploaded by the depositor.
