YCpLac111
(Plasmid
#53249)
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Purpose(Empty Backbone) contains a single replication origin (ARS1) used for plasmid replication assay
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYCpLac33
- Backbone size (bp) 5600
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Modifications to backbonesame as YCpLac33 except has LEU2 marker instead of URA3
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Vector typeUnspecified
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCpLac111 was a gift from Jean Cook (Addgene plasmid # 53249 ; http://n2t.net/addgene:53249 ; RRID:Addgene_53249) -
For your References section:
DNA replication origin function is promoted by H3K4 di-methylation in Saccharomyces cerevisiae. Rizzardi LF, Dorn ES, Strahl BD, Cook JG. Genetics. 2012 Oct;192(2):371-84. doi: 10.1534/genetics.112.142349. Epub 2012 Jul 30. 10.1534/genetics.112.142349 PubMed 22851644