pMyc3ElbLuc
(Plasmid #53245)

• Purpose: reporter plasmid containing 3 myc cDNA binding sites
• Depositing Lab: Roger Davis
• Publication: Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.

Backbone

• Vector backbone: pG5ElbLuc
• Modifications to backbone: The plasmid pG5ElbLuc was prepared from pG5E1bCAT (23) by replacing the chloramphenicol acetyltransferase gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745-bp HindIII-StuI blunt-end fragment. The GAL4 binding sites were removed from pG5ElbLuc as a Pst I and Kpn I fragment and replaced with a double-stranded 20-mer oligonucleotide that contains the Elb promoter TATA element (5'-GAGGGTATATAATGTGGTAC- 3' and 5'-CACATTATATACCCTCTGCA-3') to create the plasmid pMycOElbLuc.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pMyc3ElbLuc
• Species: H. sapiens (human)
• Mutation: To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc3ElbLuc,
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry