pMyc2ElbLuc,
(Plasmid
#53244)
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Purposereporter plasmid containing 2 myc cDNA binding sites
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53244 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepG5ElbLuc
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Modifications to backboneThe plasmid pG5ElbLuc was prepared from pG5E1bCAT (23) by replacing the chloramphenicol acetyltransferase gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745-bp HindIII-StuI blunt-end fragment. The GAL4 binding sites were removed from pG5ElbLuc as a Pst I and Kpn I fragment and replaced with a double-stranded 20-mer oligonucleotide that contains the Elb promoter TATA element (5'-GAGGGTATATAATGTGGTAC- 3' and 5'-CACATTATATACCCTCTGCA-3') to create the plasmid pMycOElbLuc.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepMyc2ElbLuc
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SpeciesH. sapiens (human)
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MutationTo prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc2ElbLuc,
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMyc2ElbLuc, was a gift from Roger Davis (Addgene plasmid # 53244 ; http://n2t.net/addgene:53244 ; RRID:Addgene_53244) -
For your References section:
Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62. Gupta S, Seth A, Davis RJ. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20. PubMed 8386367