pBS-I-Sce1-slc6a3[11kb]:egfp
(Plasmid
#53240)
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Purposeallow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 14959
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Modifications to backboneI-Sce1 addition
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Vector typecloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDAT-GFP
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)12737
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Tag
/ Fusion Protein
- GFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-I-Sce1-slc6a3[11kb]:egfp was a gift from Edward Burton (Addgene plasmid # 53240 ; http://n2t.net/addgene:53240 ; RRID:Addgene_53240) -
For your References section:
Cis-acting elements responsible for dopaminergic neuron-specific expression of zebrafish slc6a3 (dopamine transporter) in vivo are located remote from the transcriptional start site. Bai Q, Burton EA. Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12. 10.1016/j.neuroscience.2009.09.014 PubMed 19755139
Map uploaded by the depositor.
