mCer-Vamp2(qv/fw)
(Plasmid #53238)

• Purpose: Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
• Depositing Lab: Robert Zucker
• Publication: Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013.

Backbone

• Vector backbone: pECFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5063
• Modifications to backbone: This vector was created by mutagenesis of pECFP-C1. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein. See more details in Addgene's plasmid 27796.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP2
• Alt name: Vesicle-Associated Membrane Protein 2
• Alt name: synaptobrevin 2
• Species: R. norvegicus (rat)
• Insert Size (bp): 351
• Mutation: Mutated Q76 to V; F77 to W.
• GenBank ID: NM_012663
• Entrez Gene: Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
• Tags / Fusion Proteins:
  • mCerulean (N terminal on backbone)
  • 4aa-linker (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry