Stx1a-12aa-mCer
(Plasmid #53236)

• Purpose: Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
• Depositing Lab: Robert Zucker
• Publication: Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 10.1523/JNEUROSCI.2337-12.2013.

Backbone

• Vector backbone: pECFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5600
• Modifications to backbone: This vector was created by substituting ECFP in the original vector by mCerulean. Compared to ECFP, Cerulean contains mutations: S72A, Y145A and H148D. mCerulean also contains A206K mutation to create a monomeric form of the fluorescent protein.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Syntaxin 1A
• Alt name: Stx1a
• Species: R. norvegicus (rat)
• Insert Size (bp): 867
• GenBank ID: NM_053788
• Entrez Gene: Stx1a
• Tags / Fusion Proteins:
  • mCerulean (C terminal on backbone)
  • 12aa-linker (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry