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Addgene

SuperNova/pRSETB
(Plasmid #53234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
  • Alt name
    mKillerRed
  • Species
    Synthetic
  • Insert Size (bp)
    700
  • GenBank ID
    AB522905
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • Xpress tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SuperNova/pRSETB was a gift from Takeharu Nagai (Addgene plasmid # 53234 ; http://n2t.net/addgene:53234 ; RRID:Addgene_53234)

  • For your References section:

    SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T. Sci Rep. 2013;3:2629. doi: 10.1038/srep02629. 10.1038/srep02629 PubMed 24043132
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