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Addgene

pBJ-FLAG-hPar4
(Plasmid #53231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBJ
  • Backbone manufacturer
    Mark Davis lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Par4
  • Alt name
    coagulation factor II (thrombin) receptor-like 3
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of aa1-24 (native signal peptide)
  • Entrez Gene
    F2RL3 (a.k.a. PAR4)
  • Tags / Fusion Proteins
    • bovine prolactin signal sequence (MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS) (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SV40pro F
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ-FLAG-hPar4 was a gift from Shaun Coughlin (Addgene plasmid # 53231 ; http://n2t.net/addgene:53231 ; RRID:Addgene_53231)
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