pcDNA3-hPar2
(Plasmid #53228)

• Purpose: mammalian expression of human Par2
• Depositing Lab: Shaun Coughlin
• Publication: Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):1009-16. doi: 10.1042/bj3141009.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Par2
• Alt name: coagulation factor II (thrombin) receptor-like 1
• Species: H. sapiens (human)
• Entrez Gene: F2RL1 (a.k.a. GPR11, PAR2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry