pTrcHisB-TRF1
(Plasmid #53209)

• Purpose: Expressed N-terminally Hexahistidine tagged TRF1 protein
• Depositing Lab: Jack Griffith
• Publication: Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. Epub 2014 Aug 21.

Backbone

• Vector backbone: pTrcHisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4404
• Total vector size (bp): 5614
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRF1
• Alt name: TERF1
• Species: H. sapiens (human)
• Mutation: 3-439
• GenBank ID: NP_059523.2
• Entrez Gene: TERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
• Promoter: Trc
• Tag / Fusion Protein:
  • Hexahistidine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not Provided (destroyed during cloning)
• 3′ cloning site: Not Provided (destroyed during cloning)
• 5′ sequencing primer: AAT CTG TGT GGG CAC TCG
• 3′ sequencing primer: CTT CTG CGT TCT GAT TTA ATC TG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This vector is modified from vectors used by Dr. Eric Gilson in his previously published research and the TRF1 cDNA is modified from vectors used by Dr. Titia de Lange in her previously published research.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry