pTRCHisB-TRF2∆B
(Plasmid #53208)

• Purpose: Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein
• Depositing Lab: Jack Griffith
• Publication: Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. Epub 2014 Aug 21.

Backbone

• Vector backbone: pTrcHisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4404
• Total vector size (bp): 5764
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRF2 Delta Basic
• Alt name: TRF2
• Alt name: TERF2
• Species: H. sapiens (human)
• Mutation: contains amino acids 47-500
• GenBank ID: UniProtKB/Swiss-Prot: Q15554.2
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)
• Promoter: Trc
• Tag / Fusion Protein:
  • 6x His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not Provided (destroyed during cloning)
• 3′ cloning site: Not Provided (destroyed during cloning)
• 5′ sequencing primer: AAT CTG TGT GGG CAC TCG
• 3′ sequencing primer: CTT CTG CGT TCT GAT TTA ATC TG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid is modified from vectors received from Dr. Eric Gilson used in his previously published studies.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the correct TERF2 reference sequence for this plasmid is Q15554.2 (not the more recent Q15554.3 version).