pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
(Plasmid
#53207)
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PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoter
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
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Vector typeYeast genomic targeting
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAL1 promoter
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Alt nameGAL1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneGAL1 (a.k.a. YBR020W)
- Promoter GAL1 promoter
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Tags
/ Fusion Proteins
- HIS3MX6 (N terminal on insert)
- GFP (F64L, S65T, V163A) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP in Addgene plasmid #41616 was replaced with stable GFP variant (F64L, S65T, V163A).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A) was a gift from John Pringle (Addgene plasmid # 53207 ; http://n2t.net/addgene:53207 ; RRID:Addgene_53207)