pFA6a-URA3-KanMX6
(Plasmid
#53194)
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Purposegene deletion with URA3-kanMX6 double marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
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Vector typeYeast genomic targeting
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Selectable markersURA3 ; G418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameURA3-KanMX6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-URA3-KanMX6 was a gift from John Pringle (Addgene plasmid # 53194 ; http://n2t.net/addgene:53194 ; RRID:Addgene_53194) -
For your References section:
Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. Onishi M, Ko N, Nishihama R, Pringle JR. J Cell Biol. 2013 Jul 22;202(2):311-29. doi: 10.1083/jcb.201302001. 10.1083/jcb.201302001 PubMed 23878277
