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Addgene

pFA6a-VN155(I153L)-TRP1
(Plasmid #53193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-VC155-TRP1
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus
  • Species
    Synthetic
  • Mutation
    aa 1–155, I to L mutation at aa 153
  • Tag / Fusion Protein
    • TRP1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-VN155(I153L)-TRP1 was a gift from John Pringle (Addgene plasmid # 53193 ; http://n2t.net/addgene:53193 ; RRID:Addgene_53193)

  • For your References section:

    Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. Onishi M, Ko N, Nishihama R, Pringle JR. J Cell Biol. 2013 Jul 22;202(2):311-29. doi: 10.1083/jcb.201302001. 10.1083/jcb.201302001 PubMed 23878277
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