pLV hUbC-dCas9-T2A-GFP
(Plasmid #53191)

• Purpose: Co-expresses human optimized S. pyogenes dCas9 and GFP
• Depositing Lab: Charles Gersbach
• Publication: Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku749.

Backbone

• Vector backbone: FUGW
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 15000
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: humanized dead Cas9 T2A GFP
• Species: S. Pyogenes
• Mutation: D10A and H840A
• Promoter: hUbC
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Duke Limited Ancillary Agreement for dCas9
• Industry Terms:
  • Not Available to Industry