phU6-gRNA Citations (34)
Originally described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed Journal
Articles Citing phU6-gRNA
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Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA.
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
PubMed
Associated Plasmids |
| N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. Hwang PY, Jing L, Chen J, Lim FL, Tang R, Choi H, Cheung KM, Risbud MV, Gersbach CA, Guilak F, Leung VY, Setton LA. Sci Rep. 2016 Jun 13;6:28038. doi: 10.1038/srep28038. PubMed |
| Targeted Disruption of V600E-Mutant BRAF Gene by CRISPR-Cpf1. Yang M, Wei H, Wang Y, Deng J, Tang Y, Zhou L, Guo G, Tong A. Mol Ther Nucleic Acids. 2017 Sep 15;8:450-458. doi: 10.1016/j.omtn.2017.05.009. Epub 2017 May 17. PubMed |
Successful CRISPR/Cas9 mediated homologous recombination in a chicken cell line.
Antonova E, Glazova O, Gaponova A, Eremyan A, Zvereva S, Grebenkina N, Volkova N, Volchkov P.
F1000Res. 2018 Feb 28;7:238. doi: 10.12688/f1000research.13457.2. eCollection 2018.
PubMed
Associated Plasmids |
| A tetracycline-inducible CRISPR/Cas9 system, targeting two long non-coding RNAs, suppresses the malignant behavior of bladder cancer cells. Peng L, Pan P, Chen J, Yu X, Wu J, Chen Y. Oncol Lett. 2018 Oct;16(4):4309-4316. doi: 10.3892/ol.2018.9157. Epub 2018 Jul 17. PubMed |
| Optimization of CRISPR/Cas9 Delivery to Human Hematopoietic Stem and Progenitor Cells for Therapeutic Genomic Rearrangements. Lattanzi A, Meneghini V, Pavani G, Amor F, Ramadier S, Felix T, Antoniani C, Masson C, Alibeu O, Lee C, Porteus MH, Bao G, Amendola M, Mavilio F, Miccio A. Mol Ther. 2019 Jan 2;27(1):137-150. doi: 10.1016/j.ymthe.2018.10.008. Epub 2018 Oct 17. PubMed |
| Reduction of Kaposi's Sarcoma-Associated Herpesvirus Latency Using CRISPR-Cas9 To Edit the Latency-Associated Nuclear Antigen Gene. Tso FY, West JT, Wood C. J Virol. 2019 Mar 21;93(7). pii: JVI.02183-18. doi: 10.1128/JVI.02183-18. Print 2019 Apr 1. PubMed |
Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats.
Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK.
Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035.
PubMed
Associated Plasmids |
| The Crohn's disease associated SNP rs6651252 impacts MYC gene expression in human colonic epithelial cells. Matthews SM, Eshelman MA, Berg AS, Koltun WA, Yochum GS. PLoS One. 2019 Feb 22;14(2):e0212850. doi: 10.1371/journal.pone.0212850. eCollection 2019. PubMed |
| Efficient Gene Editing at Major CFTR Mutation Loci. Ruan J, Hirai H, Yang D, Ma L, Hou X, Jiang H, Wei H, Rajagopalan C, Mou H, Wang G, Zhang J, Li K, Chen YE, Sun F, Xu J. Mol Ther Nucleic Acids. 2019 Jun 7;16:73-81. doi: 10.1016/j.omtn.2019.02.006. Epub 2019 Feb 16. PubMed |
| Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Rom A, Melamed L, Gil N, Goldrich MJ, Kadir R, Golan M, Biton I, Perry RB, Ulitsky I. Nat Commun. 2019 Nov 8;10(1):5092. doi: 10.1038/s41467-019-13075-8. PubMed |
| Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients. Sullivan NT, Dampier W, Chung CH, Allen AG, Atkins A, Pirrone V, Homan G, Passic S, Williams J, Zhong W, Kercher K, Desimone M, Li L, C Antell G, Mell JC, Ehrlich GD, Szep Z, Jacobson JM, Nonnemacher MR, Wigdahl B. Sci Rep. 2019 Nov 19;9(1):17088. doi: 10.1038/s41598-019-52353-9. PubMed |
| Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function. Yoo H, Lee YJ, Park C, Son D, Choi DY, Park JH, Choi HJ, La HW, Choi YJ, Moon EH, Saur D, Chung HM, Song H, Do JT, Jang H, Lee DR, Park C, Lee OH, Cho SG, Hong SH, Kong G, Kim JH, Choi Y, Hong K. Cell Death Dis. 2020 Jan 6;11(1):14. doi: 10.1038/s41419-019-2201-1. PubMed |
| DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. Yang F, Huang X, Zang R, Chen J, Fidalgo M, Sanchez-Priego C, Yang J, Caichen A, Ma F, Macfarlan T, Wang H, Gao S, Zhou H, Wang J. Cell Stem Cell. 2020 Feb 6;26(2):234-250.e7. doi: 10.1016/j.stem.2020.01.004. PubMed |
| Reversible promoter methylation determines fluctuating expression of acute phase proteins. Zhang SC, Wang MY, Feng JR, Chang Y, Ji SR, Wu Y. Elife. 2020 Mar 30;9. pii: 51317. doi: 10.7554/eLife.51317. PubMed |
| Easy and robust electrotransfection protocol for efficient ectopic gene expression and genome editing in human B cells. Canoy RJ, Andre F, Shmakova A, Wiels J, Lipinski M, Vassetzky Y, Germini D. Gene Ther. 2020 Sep 30. pii: 10.1038/s41434-020-00194-x. doi: 10.1038/s41434-020-00194-x. PubMed |
| Direct ENIT: An easy and reliable tool for gRNA efficacy verification by tracking induced chromosomal translocation. Lomov NA, Viushkov VS, Zamalutdinov AV, Sboeva MD, Rubtsov MA. MethodsX. 2020 Oct 16;7:101104. doi: 10.1016/j.mex.2020.101104. eCollection 2020. PubMed |
| MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Ma L, Ruan J, Song J, Wen L, Yang D, Zhao J, Xia X, Chen YE, Zhang J, Xu J. Nat Commun. 2020 Nov 27;11(1):6082. doi: 10.1038/s41467-020-19842-2. PubMed |
| Profiling of H3K27Ac Reveals the Influence of Asthma on the Epigenome of the Airway Epithelium. McErlean P, Kelly A, Dhariwal J, Kirtland M, Watson J, Ranz I, Smith J, Saxena A, Cousins DJ, Van Oosterhout A, Solari R, Edwards MR, Johnston SL, Lavender P. Front Genet. 2020 Dec 10;11:585746. doi: 10.3389/fgene.2020.585746. eCollection 2020. PubMed |
| Improved efficiency of genome editing by constitutive expression of Cas9 endonuclease in genetically-modified mice. Ghassemi B, Jamalkhah M, Shokri G, Kehtari M, Soleimani M, Shamsara M, Kiani J. 3 Biotech. 2021 Feb;11(2):56. doi: 10.1007/s13205-020-02580-z. Epub 2021 Jan 11. PubMed |
| Two redundant ubiquitin-dependent pathways of BRCA1 localization to DNA damage sites. Sherker A, Chaudhary N, Adam S, Heijink AM, Noordermeer SM, Fradet-Turcotte A, Durocher D. EMBO Rep. 2021 Dec 6;22(12):e53679. doi: 10.15252/embr.202153679. Epub 2021 Nov 2. PubMed |
| Mutations of the histone linker H1-4 in neurodevelopmental disorders and functional characterization of neurons expressing C-terminus frameshift mutant H1.4. Tremblay MW, Green MV, Goldstein BM, Aldridge AI, Rosenfeld JA, Streff H, Tan WD, Craigen W, Bekheirnia N, Al Tala S, West AE, Jiang YH. Hum Mol Genet. 2021 Nov 12. pii: 6414843. doi: 10.1093/hmg/ddab321. PubMed |
| PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed |
CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells.
Werder RB, Liu T, Abo KM, Lindstrom-Vautrin J, Villacorta-Martin C, Huang J, Hinds A, Boyer N, Bullitt E, Liesa M, Silverman EK, Kotton DN, Cho MH, Zhou X, Wilson AA.
Sci Adv. 2022 Jul 15;8(28):eabo6566. doi: 10.1126/sciadv.abo6566. Epub 2022 Jul 13.
PubMed
Associated Plasmids |
| Transcriptional regulation of Satb1 in mouse trophoblast stem cells. Yu W, Chakravarthi VP, Borosha S, Dilower I, Lee EB, Ratri A, Starks RR, Fields PE, Wolfe MW, Faruque MO, Tuteja G, Rumi MAK. Front Cell Dev Biol. 2022 Dec 14;10:918235. doi: 10.3389/fcell.2022.918235. eCollection 2022. PubMed |
| Bi-allelic ATG4D variants are associated with a neurodevelopmental disorder characterized by speech and motor impairment. Morimoto M, Bhambhani V, Gazzaz N, Davids M, Sathiyaseelan P, Macnamara EF, Lange J, Lehman A, Zerfas PM, Murphy JL, Acosta MT, Wang C, Alderman E, Reichert S, Thurm A, Adams DR, Introne WJ, Gorski SM, Boerkoel CF, Gahl WA, Tifft CJ, Malicdan MCV. NPJ Genom Med. 2023 Feb 10;8(1):4. doi: 10.1038/s41525-022-00343-8. PubMed |
| TROP2 translation mediated by dual m(6)A/m(7)G RNA modifications promotes bladder cancer development. Chen C, Chao Y, Zhang C, Hu W, Huang Y, Lv Y, Liu B, Ji D, Liu M, Yang B, Jiang L, Liang Y, Zhang H, Yuan G, Ying X, Ji W. Cancer Lett. 2023 Jul 10;566:216246. doi: 10.1016/j.canlet.2023.216246. Epub 2023 Jun 1. PubMed |
| An AlphaFold2 map of the 53BP1 pathway identifies a direct SHLD3-RIF1 interaction critical for shieldin activity. Sifri C, Hoeg L, Durocher D, Setiaputra D. EMBO Rep. 2023 Aug 3;24(8):e56834. doi: 10.15252/embr.202356834. Epub 2023 Jun 12. PubMed |
| Allelic chromatin structure primes imprinted expression of Kcnk9 during neurogenesis. Loftus D, Bae B, Whilden CM, Whipple AJ. bioRxiv. 2023 Jun 9:2023.06.09.544389. doi: 10.1101/2023.06.09.544389. Preprint. PubMed |
| Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy. Grosch M, Schraft L, Chan A, Kuchenhoff L, Rapti K, Ferreira AM, Kornienko J, Li S, Radke MH, Kramer C, Clauder-Munster S, Perlas E, Backs J, Gotthardt M, Dieterich C, van den Hoogenhof MMG, Grimm D, Steinmetz LM. Nat Commun. 2023 Jun 22;14(1):3714. doi: 10.1038/s41467-023-39352-1. PubMed |
| Specificity of cancer-related chromosomal translocations is linked to proximity after the DNA double-strand break and subsequent selection. Canoy RJ, Shmakova A, Karpukhina A, Lomov N, Tiukacheva E, Kozhevnikova Y, Andre F, Germini D, Vassetzky Y. NAR Cancer. 2023 Sep 23;5(3):zcad049. doi: 10.1093/narcan/zcad049. eCollection 2023 Sep. PubMed |
Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells.
Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M.
Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0.
PubMed
Associated Plasmids |
| CRISPR/Cas9 model of prostate cancer identifies Kmt2c deficiency as a metastatic driver by Odam/Cabs1 gene cluster expression. Cai H, Zhang B, Ahrenfeldt J, Joseph JV, Riedel M, Gao Z, Thomsen SK, Christensen DS, Bak RO, Hager H, Vendelbo MH, Gao X, Birkbak N, Thomsen MK. Nat Commun. 2024 Mar 7;15(1):2088. doi: 10.1038/s41467-024-46370-0. PubMed |
| Exchange of subtelomeric regions between chromosomes 4q and 10q reverts the FSHD genotype and phenotype. Ma Y, Schwager Karpukhina A, Dib C, Gautier C, Hermine O, Allemand E, Vassetzky YS. Sci Adv. 2024 May 3;10(18):eadl1922. doi: 10.1126/sciadv.adl1922. Epub 2024 May 1. PubMed |
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