pmU6-gRNA Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid
53187 with the following enzymes: PvuI single digest and PvuI and PciI double digest
We expect the linearized plasmid to run at ~3.6kb and the double digest to give 2 bands of ~2.5 and 1.1kb. We see the expected pattern, although the 1.1 kb band is too light to visualize on this image.
