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Addgene

pFA6a-GFP (F64L, S65T, V163A)-His3MX6
(Plasmid #53185)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIs3MX6
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HIS3 (a.k.a. YOR202W, HIS10, HIS8)
  • Tag / Fusion Protein
    • GFP (F64L, S65T, V163A) (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is similar to Addgene plasmid #41598, except the GFP has been switched to a more stable variant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-GFP (F64L, S65T, V163A)-His3MX6 was a gift from John Pringle (Addgene plasmid # 53185 ; http://n2t.net/addgene:53185 ; RRID:Addgene_53185)

  • For your References section:

    Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis. Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, Pringle JR. Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17. 10.1091/mbc.e07-05-0509 PubMed 17942599
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