pFA6a-GFP (F64L, S65T, V163A)-TRP1
(Plasmid
#53184)
-
Purposechromosomal tagging with stable GFP
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepFA6a
-
Vector typeYeast genomic targeting
-
Selectable markersTRP1
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTRP1
-
SpeciesS. cerevisiae (budding yeast)
-
Entrez GeneTRP1 (a.k.a. YDR007W)
-
Tag
/ Fusion Protein
- GFP (F64L, S65T, V163A) (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is similar to Addgene plasmid #41597, except the GFP has been switched to a more stable variant.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a-GFP (F64L, S65T, V163A)-TRP1 was a gift from John Pringle (Addgene plasmid # 53184 ; http://n2t.net/addgene:53184 ; RRID:Addgene_53184) -
For your References section:
Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis. Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, Pringle JR. Mol Biol Cell. 2007 Dec;18(12):5139-53. Epub 2007 Oct 17. 10.1091/mbc.e07-05-0509 PubMed 17942599