pCSaE500
(Plasmid
#53182)
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PurposeInducible expression of E gene which produces a lysis protein that induces autolysis.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDFDuet-1
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Backbone manufacturerNovagen
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepCSaE500
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Alt namepCSaE
- Promoter ampR-PampC cassette
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5' - TCC TGG GTC TGT TTA GTG G - 3'; 5' - TCC CTT ATG CGA CTC CTG - 3'
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSaE500 was a gift from Lingchong You (Addgene plasmid # 53182 ; http://n2t.net/addgene:53182 ; RRID:Addgene_53182) -
For your References section:
Programming stress-induced altruistic death in engineered bacteria. Tanouchi Y, Pai A, Buchler NE, You L. Mol Syst Biol. 2012;8:626. doi: 10.1038/msb.2012.57. 10.1038/msb.2012.57 PubMed 23169002
Map uploaded by the depositor.
