pWZLblasti-HA-ERK2 R67S
(Plasmid #53172)

• Purpose: Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector
• Depositing Lab: Christopher Counter
• Publication: Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature13180.

Backbone

• Vector backbone: pWZLblasti
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERK2
• Alt name: Extracellular signal regulated kinase 2
• Alt name: MAPK1
• Alt name: Mitogen activated protein kinase 2
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 1082
• Mutation: R67S
• GenBank ID: NM_138957.2
• Entrez Gene: MAPK1 (a.k.a. ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAACCTCCTCGTTCGACC
• 3′ sequencing primer: TTCCGGGCCCTCACATTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid 23498: pDONR223-MAPK1
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry