pWZLblasti-HA-MEK1
(Plasmid #53161)

• Purpose: Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector
• Depositing Lab: Christopher Counter
• Publication: Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature13180.

Backbone

• Vector backbone: pWZLblasti
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 6281
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEK1
• Alt name: MAP2K1
• Alt name: MAPK/ERK kinase 1
• Alt name: Dual specificity mitogen activated protein kinase kinase 1
• Species: H. sapiens (human)
• Insert Size (bp): 1181
• GenBank ID: NM_002755.3 NP_002746.1
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAACCTCCTCGTTCGACC
• 3′ sequencing primer: TTCCGGGCCCTCACATTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Plasmid 21208: W1
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry