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pWZLblasti-CTR1M154A
(Plasmid #53158)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZLblasti
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 5672
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTR1
  • Alt name
    Copper transporter 1
  • Alt name
    Solute carrier family 31 (copper tansporters), member 1
  • Alt name
    COPT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    572
  • Mutation
    M154A
  • GenBank ID
    NM_001859.3
  • Entrez Gene
    SLC31A1 (a.k.a. COPT1, CTR1, NSCT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GAACCTCCTCGTTCGACC
  • 3′ sequencing primer TTCCGGGCCCTCACATTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The M81L mutation found during the QC process has no functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZLblasti-CTR1M154A was a gift from Christopher Counter (Addgene plasmid # 53158 ; http://n2t.net/addgene:53158 ; RRID:Addgene_53158)

  • For your References section:

    Copper is required for oncogenic BRAF signalling and tumorigenesis. Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ, Counter CM. Nature. 2014 Apr 9. doi: 10.1038/nature13180. 10.1038/nature13180 PubMed 24717435
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