pWZLblasti-CTR1
(Plasmid #53157)

• Purpose: Expresses human CTR1 from blasticidin resistance retroviral vector
• Depositing Lab: Christopher Counter
• Publication: Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature13180.

Backbone

• Vector backbone: pWZLblasti
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 5672
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTR1
• Alt name: Copper transporter 1
• Alt name: Solute carrier family 31 (copper tansporters), member 1
• Alt name: COPT1
• Species: H. sapiens (human)
• Insert Size (bp): 572
• GenBank ID: NM_001859.3
• Entrez Gene: SLC31A1 (a.k.a. COPT1, CTR1, NSCT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAACCTCCTCGTTCGACC
• 3′ sequencing primer: TTCCGGGCCCTCACATTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The M40V mutation found during the QC process has no functional consequence.