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Addgene

pcDNA3.1 SIRT7_H187Y
(Plasmid #53151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sirt7
  • Alt name
    sirtuin 7
  • Species
    H. sapiens (human)
  • Mutation
    H187Y, catalytic inactive
  • GenBank ID
  • Entrez Gene
    SIRT7 (a.k.a. SIR2L7)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 SIRT7_H187Y was a gift from Katrin Chua (Addgene plasmid # 53151 ; http://n2t.net/addgene:53151 ; RRID:Addgene_53151)

  • For your References section:

    SIRT7 represses Myc activity to suppress ER stress and prevent fatty liver disease. Shin J, He M, Liu Y, Paredes S, Villanova L, Brown K, Qiu X, Nabavi N, Mohrin M, Wojnoonski K, Li P, Cheng HL, Murphy AJ, Valenzuela DM, Luo H, Kapahi P, Krauss R, Mostoslavsky R, Yancopoulos GD, Alt FW, Chua KF, Chen D. Cell Rep. 2013 Nov 14;5(3):654-65. doi: 10.1016/j.celrep.2013.10.007. Epub 2013 Nov 7. 10.1016/j.celrep.2013.10.007 PubMed 24210820
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