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Addgene

pSUPERretro-Sirt6 shRNA1
(Plasmid #53147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPERretro
  • Backbone manufacturer
    OligoEngine
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sirt6
  • Alt name
    sirtuin 6
  • gRNA/shRNA sequence
    AAGCTGGAGCCCAAGGAGGAA
  • Promoter H1 RNA polymerase III

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pLXSN5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPERretro-Sirt6 shRNA1 was a gift from Katrin Chua (Addgene plasmid # 53147 ; http://n2t.net/addgene:53147 ; RRID:Addgene_53147)

  • For your References section:

    SIRT6 is required for maintenance of telomere position effect in human cells. Tennen RI, Bua DJ, Wright WE, Chua KF. Nat Commun. 2011 Aug 16;2:433. doi: 10.1038/ncomms1443. 10.1038/ncomms1443 PubMed 21847107
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