pET11a-SUMO3
(Plasmid #53143)

• Purpose: E.coli expression of untagged mature human SUMO3 (amino acids 1-93)
• Depositing Lab: Frauke Melchior
• Publication: Flotho et al Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.

Backbone

• Vector backbone: pET11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5677
• Total vector size (bp): 5922
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUMO3
• Alt name: SUMO2
• Alt name: Smt3B
• Species: H. sapiens (human)
• Insert Size (bp): 282
• Mutation: mature form (amino acids 1-93)
• GenBank ID: Uniprot P61956
• Entrez Gene: SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • SUMO nomenclature
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry