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Addgene

pET11a-SUMO2
(Plasmid #53142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5677
  • Total vector size (bp) 5919
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUMO2
  • Alt name
    Smt3A
  • Alt name
    SUMO3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    279
  • Mutation
    mature form (amino acids 1-92)
  • GenBank ID
    Uniprot P55854
  • Entrez Gene
    SUMO3 (a.k.a. SMT3A, SMT3H1, SUMO-3, Smt3B)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11a-SUMO2 was a gift from Frauke Melchior (Addgene plasmid # 53142 ; http://n2t.net/addgene:53142 ; RRID:Addgene_53142)

  • For your References section:

    Recombinant reconstitution of sumoylation reactions in vitro. Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F. Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5. 10.1007/978-1-61779-474-2_5 PubMed 22350878
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