pET28a-HisYFP-Sp100
(Plasmid #53141)

• Purpose: E.coli expression of HisYFP tagged human Sp100 fragment
• Depositing Lab: Frauke Melchior
• Publication: Flotho et al Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7283
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Sp100A fragment
• Species: H. sapiens (human)
• Insert Size (bp): 2028
• Mutation: fragment (amino acids 71-480); deletion of L75
• GenBank ID:
• Entrez Gene: SP100 (a.k.a. lysp100b)
• Promoter: T7
• Tag / Fusion Protein:
  • His-YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid contains some 3'UTR sequence following the CDS that is not found in the full plasmid sequence. This does not have any functional consequence.