pGEX3x-hRanBP2-(IR1+M)
(Plasmid #53140)

• Purpose: E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity
• Depositing Lab: Frauke Melchior
• Publication: Flotho et al Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.

Backbone

• Vector backbone: pGEX-3X
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 5577
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RanBP2 fragment
• Alt name: Nup358
• Species: H. sapiens (human)
• Insert Size (bp): 641
• Mutation: fragment (amino acids 2631-2711)
• Entrez Gene: RANBP2 (a.k.a. ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5-FP
• 3′ sequencing primer: pGEX3-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry