pET23a-His-hRanGAP1tail
(Plasmid #53139)

• Purpose: E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag
• Depositing Lab: Frauke Melchior
• Publication: Flotho et al Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.

Backbone

• Vector backbone: pET23a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4196
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RanGAP1
• Alt name: Ran GTPase activating protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 606
• Mutation: C-terminal fragment (amino acids 398-587)
• GenBank ID:
• Entrez Gene: RANGAP1 (a.k.a. Fug1, RANGAP, SD)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry