pET11a-SUMO1
(Plasmid #53138)

• Purpose: E.coli expression of untagged mature human SUMO1 (amino acids 1-97)
• Depositing Lab: Frauke Melchior
• Publication: Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Backbone

• Vector backbone: pET11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5677
• Total vector size (bp): 5993
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUMO1
• Alt name: SUMO1 small ubiquitin-like modifier 1
• Species: H. sapiens (human)
• Insert Size (bp): 294
• Mutation: mature form (amino acids 1-97)
• GenBank ID:
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry