pET23a-mUbc9
(Plasmid #53137)

• Purpose: E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)
• Depositing Lab: Frauke Melchior
• Publication: Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4107
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubc9
• Alt name: UBE2I
• Species: M. musculus (mouse)
• Insert Size (bp): 477
• Mutation: protein sequence is identical to human
• Entrez Gene: Ube2i (a.k.a. 5830467E05Rik, UBC9, Ubce2i, Ubce9)
• Promoter: T7
• Tag / Fusion Protein:
  • untagged

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pET-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry