pET11d-hUba2
(Plasmid #53136)

• Purpose: E.coli expression of full length untagged human Uba2 (SAE2)
• Depositing Lab: Frauke Melchior
• Publication: Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Backbone

• Vector backbone: pET11d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5674
• Total vector size (bp): 7574
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: UBA2
• Alt name: SAE2
• Alt name: SUMO-activating enzyme subunit 2
• Species: H. sapiens (human)
• Insert Size (bp): 1942
• GenBank ID:
• Entrez Gene: UBA2 (a.k.a. ACCES, ARX, HRIHFB2115, SAE2)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry