pET28a-His-hAos1
(Plasmid #53135)

• Purpose: E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag
• Depositing Lab: Frauke Melchior
• Publication: Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6496
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Aos1
• Alt name: SAE1
• Alt name: SUMO-activating enzyme subunit 1
• Species: H. sapiens (human)
• Insert Size (bp): 1166
• GenBank ID:
• Entrez Gene: SAE1 (a.k.a. AOS1, HSPC140, SUA1, UBLE1A)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry