pGfa2-luc3
(Plasmid #53129)

• Purpose: luciferase reporter for human gfa2 promoter
• Depositing Lab: Michael Brenner
• Publication: Brenner lab unpublished plasmids (unpublished)

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Total vector size (bp): 7049
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gfa2 promoter fragment
• Alt name: gfa2
• Alt name: glial fibrillary acidic protein
• Species: H. sapiens (human)
• Mutation: contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG
• Entrez Gene: GFAP (a.k.a. ALXDRD)
• Promoter: gfa2
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNRev

Resource Information

• Supplemental Documents:
  • pGfa2-luc3 sequence with annotations

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Bgl II/Hind III gfa2 promoter fragment is from pGfa-CAT2 (for construction of pGfa-CAT2, see Besnard et al. 1991).