SNV-memTdTomato-2AcytoGFP
(Plasmid #53124)

• Purpose: (Empty Backbone) Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
• Depositing Lab: Jeanette Hyer
• Publication: Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766.

Backbone

• Vector backbone: pBMN-LACZ
• Backbone manufacturer: Nolan Lab
• Vector type: Retroviral ; expression in chicken cells
• Promoter: SNV (spleen necrosis virus)
• Tags / Fusion Proteins:
  • memTdTomato (C terminal on insert)
  • 2A-cyto-GFP (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBMN5'
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest before proceeding further (~9kb).