SNV-memTdTomato
(Plasmid
#53123)
-
Purposeretroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 53123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBMN-LACZ
-
Backbone manufacturerNolan Lab
-
Modifications to backboneSNV promoter was added.
-
Vector typeRetroviral ; expression in chicken cells
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namememTdTomato
-
SpeciesSynthetic
- Promoter SNV (spleen necrosis virus)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBMN 5'
- 3′ sequencing primer LNCX-3 (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is not used as a cloning vector.
This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest before proceeding further (~7kb).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SNV-memTdTomato was a gift from Jeanette Hyer (Addgene plasmid # 53123 ; http://n2t.net/addgene:53123 ; RRID:Addgene_53123) -
For your References section:
Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo. Venters SJ, Dias da Silva MR, Hyer J. Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766. 10.1002/dvdy.21766 PubMed 18942139