pLenti-OC-IRES-BSD
(Plasmid #53118)

• Purpose: To create stable cell clones with high-level expression of Cas9 and OCT1
• Depositing Lab: Wensheng Wei
• Publication: Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature13166.

Backbone

• Vector backbone: pLenti-CMV-BSD
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: OCT1
• Alt name: POU class 2 homeobox 1
• Alt name: POU2F1
• Species: H. sapiens (human)
• Entrez Gene: POU2F1 (a.k.a. OCT1, OTF1, Oct1Z, oct-1B)
• Promoter: CMV
• Tag / Fusion Protein:
  • P2A (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Gene/Insert 2

• Gene/Insert name: Cas9
• Species: Streptococcus pyogenes
• Mutation: human codon-optimized
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • IRES-BSD (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: pCDH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry