pET28b-hUba2-His
(Plasmid #53117)

• Purpose: expression of full length human Uba2 with Cterminal His-tag in E.coli
• Depositing Lab: Frauke Melchior
• Publication: Werner et al Methods Mol Biol. 2009;497:187-99. doi: 10.1007/978-1-59745-566-4_12.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7234
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: UBA2
• Alt name: SAE2
• Alt name: SUMO-activating enzyme subunit 2
• Species: H. sapiens (human)
• Insert Size (bp): 1937
• GenBank ID:
• Entrez Gene: UBA2 (a.k.a. ACCES, ARX, HRIHFB2115, SAE2)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (C terminal on insert)
  • T7 tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry