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Addgene

JF001A
(Plasmid #53116)

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Item Catalog # Description Quantity Price (USD)
Plasmid 53116 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5442
  • Total vector size (bp) 6278
  • Modifications to backbone
    lacO and original RBS were removed from pET21b and substituted with the theophylline riboswitch
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    theophylline riboswitch and alpha hemolysin
  • Alt name
    alpha hemolysin from Staphylococcus aureus
  • Alt name
    αHL
  • Species
    Staphylococcus aureus
  • Insert Size (bp)
    939
  • Promoter T7 promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GGCAGCACCCTGCTAAGGTAACAACAAG ATGGATTCTGATATCAATATCAAAACC
  • 3′ sequencing primer AAG GGC ATC AAG ACG ATG CTG GTA TCA CCC TAT AGT GAG TCG TAT TAA TTT CGC GG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JF001A was a gift from Sheref Mansy (Addgene plasmid # 53116 ; http://n2t.net/addgene:53116 ; RRID:Addgene_53116)

  • For your References section:

    Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour. Lentini R, Santero SP, Chizzolini F, Cecchi D, Fontana J, Marchioretto M, Del Bianco C, Terrell JL, Spencer AC, Martini L, Forlin M, Assfalg M, Dalla Serra M, Bentley WE, Mansy SS. Nat Commun. 2014 May 30;5:4012. doi: 10.1038/ncomms5012. 10.1038/ncomms5012 PubMed 24874202
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