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Addgene

pCMVTNT-EPHX1
(Plasmid #53114)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53114 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVTNT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4050
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPHX1
  • Alt name
    MEH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1368
  • GenBank ID
    NM_000120
  • Entrez Gene
    EPHX1 (a.k.a. EPHX, EPOX, HYL1, MEH)
  • Promoter CMV and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVTNT-EPHX1 was a gift from Xu Lin (Addgene plasmid # 53114 ; http://n2t.net/addgene:53114 ; RRID:Addgene_53114)

  • For your References section:

    Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects of benzo [alpha] pyrene by interacting with microsomal epoxide hydrolase and enhancing its hydrolysis activity. Chen JY, Chen WN, Jiao BY, Lin WS, Wu YL, Liu LL, Lin X. BMC Cancer. 2014 Apr 23;14:282. doi: 10.1186/1471-2407-14-282. 10.1186/1471-2407-14-282 PubMed 24758376
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